Dec 20, 2017 · This review summarizes the mechanisms of the initiator protein DnaA in replication initiation and its regulation in Escherichia coli. The chromosomal origin (oriC) DNA …

DnaA protein, the initiator of chromosomal replication in Escherichia coli, is purified as a soluble protein, and in vitro it efficiently initiates replication of minichromosomes in membrane-free …

The oriC site in E. coli has three A T rich 13 base pair regions followed by four 9 bp regions with the sequence TTAT(C or A)CA(C or A)A. [5] DnaA molecules bind to the 9 bp regions, which …

Dec 21, 2017 · DiaA binds to DnaA and stimulates DnaA assembly at the DOR. DnaA binds tightly to ATP and ADP. ATP-DnaA constructs functionally different sub-complexes at DOR, …

Nov 1, 2002 · The Synechocystis DnaA protein binds to DnaA boxes, as does its C-terminal domain 4 with affinities comparable to E. coli DnaA. The genome of Synechocystis has been …

To initiate DNA replication, DnaA recognizes and binds to specific sequences within the Escherichia coli chromosomal origin (oriC), and then unwinds a region within oriC. Next, DnaA …

In E. coli, replication initiation is regulated by various regulatory systems during the cell cycle. The main targets of these systems are oriC and DnaA.

Here, we use a ChIP-seq to map the E. coli DnaA-binding landscape. Our data reveal a compact regulon for DnaA that coordinates the initiation of DNA replication with expression of genes …

In Escherichia coli, DnaA protein is the functional counterpart to ORC in that it recognizes specific sequences in the replication origin, oriC. Once bound, it directs formation of the initiation …

Sep 1, 2009 · Escherichia coli DnaA is the initiator of chromosomal replication. Multiple ATP-DnaA molecules assemble at the oriC replication origin in a highly regulated manner, and the …