After resuspending the samples in fresh blocking/trituration media, tissue was passed through a series of decreasing bore size, fire-polished Pasteur pipettes. The dissociated cells were pelleted by ...
After carefully removing the aqueous phase using a pipette into another 1.5 mL Eppendorf tube, some of the aqueous phase (about 1 mm above DNA layer to prevent DNA contamination) was remained. 550 µL ...
We homogenized the sample by repeated pipetting using a P-200 pipette tip and then followed the manufacturer’s instructions for phenol–chloroform extraction and ethanol precipitation to purify total ...
In their important manuscript, Costa et al. establish an in vitro model for dorsal root ganglion (DRG) axonal asymmetry, revealing that central and peripheral axon branches have distinct patterns of ...
Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN) and Advanced Materials Bio-Engineering Research Centre (AMBER), Trinity College Dublin, Dublin 2, Ireland ...
Sharps include all objects that can penetrate the skin, such as: ...
Biological Safety Cabinets (BSCs), or biosafety cabinets, are primary containment devices utilized in laboratories for the handling of biohazardous agents. They are routinely used for a wide variety ...
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