1| Prepare a 40-ml 4.5% native acrylamide gel (using 1 to 1.5-mm spacers): Prerun the 'gel for 2 h at 10 mA. Large protein complexes greater than 1 MDa in mass are difficult to resolve and migrate ...

While researchers have long relied on electrophoretic mobility-shift assays (called gel shifts) to identify which transcription factors bind to DNA samples, the method can be time-consuming and ...

Scientists who study protein-DNA interactions frequently rely upon gel shift assays or electrophoretic mobility shift assays (EMSAs), which are based on relative differences in electrophoretic ...

The expression of these five genes was directly activated by SmMYB113 through yeast one-hybrid, electrophoretic mobility shift assay, and dual luciferase experiments. Overall, the research not ...