1| Prepare a 40-ml 4.5% native acrylamide gel (using 1 to 1.5-mm spacers): Prerun the 'gel for 2 h at 10 mA. Large protein complexes greater than 1 MDa in mass are difficult to resolve and migrate ...

The expression of these five genes was directly activated by SmMYB113 through yeast one-hybrid, electrophoretic mobility shift assay, and dual luciferase experiments. Overall, the research not ...